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about antibody Extinction Coefficient

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#1 Sherry Zhu

Sherry Zhu


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Posted 29 October 2010 - 06:55 AM

Hello everyone:

I have a question about Extinction Coefficient.

I use the tool on http://www.expasy.ch/ and the software calculate the Extinction Coefficient and absorbance (optical density) ,using the following formula: Absorb(Prot) = E(Prot) / Molecular_weight. The Molecular weight in the formula is deduced from the amimo acid sequence I input. However, my sample is a kind of monoantibody which has two glycosyl-chains, the actual MW is bigger than the deduced MW. The software may forget this modification! Now I am confused and I don't know which MW should be used as Molecular_weight in the formula, the deduced value or the actual one? And I am also doubt if the glycosylation has effect on Extinction Coefficient.

Thank you! :D

Edited by Sherry Zhu, 29 October 2010 - 07:02 AM.

#2 sgt4boston



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Posted 01 November 2010 - 02:36 AM

Read some of the posting under "Antibody Concentration" at the top of the list...it may be of help to you.

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