Hi,
I need to prepare buffer for ELISA, that is possibly closest to PBS, but has pH 8,6. The most important is ion strenth of ions Na+ and K+. Is it possible to keep salts with PO4 3-?
I would be gratefull for any infos,
Greets!
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mdfenko
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Excellent
Excellent
Posted 29 October 2010 - 07:34 AM
phosphate will not be an effective buffer at pH 8.6. which buffer you use depends on which direction of pH change you need to resist.
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