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How to make a buffer similar to PBS, pH 8,6?


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#1 bithorax

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Posted 29 October 2010 - 04:38 AM

Hi,
I need to prepare buffer for ELISA, that is possibly closest to PBS, but has pH 8,6. The most important is ion strenth of ions Na+ and K+. Is it possible to keep salts with PO4 3-?
I would be gratefull for any infos,
Greets!

#2 mdfenko

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Posted 29 October 2010 - 07:34 AM

phosphate will not be an effective buffer at pH 8.6. which buffer you use depends on which direction of pH change you need to resist.
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