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DNA yields after Bisulfite Treatment


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15 replies to this topic

#16 pcrman

pcrman

    Epigenetist

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Posted 08 July 2014 - 09:15 AM

Hi, I am new to this forum and doing BS conversion with Epitech Bisulfite conversion kit. I am using 10ug DNA but i am getting only 50ng DNA after BS conversion. I am doing the quantification with Qubit  from Invitrogen.  Please suggest me to get high amount of DNA after conversion.

 

Thanx

Hi Rama,

 

I think you cannot use too much starting DNA in order to get high DNA yield. What amount of DNA does the kit suggests you to use? Using too much starting DNA can lead to incomplete conversion of unmethylated cytosines to uracils.   We don't usually measure DNA concentration after conversion, and to see amplification, we do two rounds of PCRs.






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