Storage of Bacterial Culture at 4 degrees
Posted 28 October 2010 - 01:39 AM
I faced a problem. After pelleting down the bacterial culture, the pellet wasn't hard, as it used to be. It gave a hazy, hairy appearance. After discarding the supernatant, I added TE buffer to re-suspend the pellet, the pellet could not be re-suspended. It remained a pellet.
Can any1 explain what happened? Is it advisable to store bacterial cultures at 4 degrees, or is the problem due to other reasons?
Posted 28 October 2010 - 02:52 AM
other than that, no idea, sounds strange to me, in general I think next time if you want to leave the culture overnight the usual recommendation is to spin it down to a pellet, discard supernatant then store at -80 I think.
Posted 28 October 2010 - 08:38 PM
"hazy, hairy appearance."
It could be that the culture has began to flocculate.
However it could also mean that the culture was contaminated. Does the culture smell different or has a different colour compared to a normal e coli culture.