confusion in real time PCR primers
Posted 27 October 2010 - 06:03 PM
i am new to this REAL TIME PCR.i need to work on a gene. i was going through the papers for primers each primer sequence differ from other in different papers for the same gene.I dont understand why this difference for the same gene and i dnt know which one i need to select?
i need to do with EVA green not probe based. kindly help me in this.
i hope you people will help me on this.....
Posted 02 December 2010 - 06:49 AM
Use primer3 software and have a try!
Posted 07 December 2010 - 03:32 PM
Posted 10 December 2010 - 04:41 PM