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rna isolation


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#1 deespike

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Posted 27 October 2010 - 08:30 AM

Hi all

I am isolating rna from leaf of plant and I am always in problem regarding the grinding of the tissue properly. I use liquid nitrogen for grinding the tissue but i am very much interested to know whether there is any method like where i dont need to use liquid nitrogen and still get my work done- homogenization?

I have read of bead based homogenizers, does any one have any idea whether these require liquid nitrogen?
Plz help.

#2 Curtis

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Posted 29 October 2010 - 07:44 AM

I'm not really familiar with plant biology, but is this is a common conventional method or are you using a kit?




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