Problem with Qiagen EpiTect Bisulfite Kit
Posted 26 October 2010 - 11:01 AM
I am using Qiagen EpiTect Bisulfite Kit for detect methylation status.
I isolated DNA, quantified it, and I did PCR assay.
I have only amplification in PCR with primers for DNA methylated.
I have not amplification in PCR with primers for DNA no methylated.
I think the problem is in bisulfite treatment.
Where is the problem???
Posted 26 October 2010 - 11:47 AM
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 29 October 2010 - 11:22 PM
are you expecting your samples to be unmethylated or methylated? does sound like your conversion has worked.
Thank you! yesterday i had good results!!!!!! the problem was in methylation assay.