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Problem with Qiagen EpiTect Bisulfite Kit


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#1 b612

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Posted 26 October 2010 - 11:01 AM

Hi!
I am using Qiagen EpiTect Bisulfite Kit for detect methylation status.
I isolated DNA, quantified it, and I did PCR assay.
I have only amplification in PCR with primers for DNA methylated.
I have not amplification in PCR with primers for DNA no methylated.
I think the problem is in bisulfite treatment.
Please!!!
Help!!!
Where is the problem???

#2 methylnick

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Posted 26 October 2010 - 11:47 AM

are you expecting your samples to be unmethylated or methylated? does sound like your conversion has worked.

Nick

#3 b612

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Posted 29 October 2010 - 11:22 PM

are you expecting your samples to be unmethylated or methylated? does sound like your conversion has worked.

Nick



Thank you! yesterday i had good results!!!!!! the problem was in methylation assay.




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