3 replies to this topic

[fredUNH]

Hi all, I'm going to fractionate mouse skeletal muscle using Focus Subcell kit. In order to facilitate the organization of the experiment, Do you know if a post mitochondrial (cytosolic) fraction can be stored or freezed until further centrifugation at 100,000g? it contains cytosol and membranes which I want to separate for protein analyses.
Thank you in advance,

Fred

[Curtis]

yes, store at -80C

[rhombus]

fredUNH, on 25 October 2010 - 12:47 AM, said:

Hi all, I'm going to fractionate mouse skeletal muscle using Focus Subcell kit. In order to facilitate the organization of the experiment, Do you know if a post mitochondrial (cytosolic) fraction can be stored or freezed until further centrifugation at 100,000g? it contains cytosol and membranes which I want to separate for protein analyses.
Thank you in advance,

Fred

Does the kit supply a buffer that contains a protease/phosphatase inhibitor cocktail. Using inhibitors will reduce degradation of proteins and will keep the cytosolic fraction in good condition for longer storage at -80oC.

Kindest regards.

Neale.

[fredUNH]

I did not have any confirmation of your answers! sorry for this late acknowledgement !!