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midiprep


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#1 SF_HK

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Posted 24 October 2010 - 11:14 PM

Hi,

I've eluted my plasmid using midi-prep and the concentration is 1800ng/ul. I need to trafect my cells with 6ug using lipofectamine. Just out of curiosity, is there a problem using too concentarted plasmid?

#2 hematopoietry

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Posted 26 October 2010 - 02:20 AM

Hi,

I've eluted my plasmid using midi-prep and the concentration is 1800ng/ul. I need to trafect my cells with 6ug using lipofectamine. Just out of curiosity, is there a problem using too concentarted plasmid?


According to invitrogen manual (http://tools.invitro...ine2000_man.pdf) you will add LFM when you diluted DNA to a certain concentration. It works similar for other manufacturers. If you follow the protocol you should be good;)




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