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problem with MACS cell isolation


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#1 tantao

tantao

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Posted 24 October 2010 - 11:10 PM

Dear everyone,
we use MACS to isolate the positive cells.But after isolation the positive cells are all in the depletion part. I do not know why, and someone told me if the concentartion of primary antibody is too low or high. The MACS does not work! IS that true? Shall I titre the antibody? Thanks very much!




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