we use MACS to isolate the positive cells.But after isolation the positive cells are all in the depletion part. I do not know why, and someone told me if the concentartion of primary antibody is too low or high. The MACS does not work! IS that true? Shall I titre the antibody? Thanks very much!
Submit your paper to J Biol Methods today!
problem with MACS cell isolation
No replies to this topic