Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

QPCR output generates no Cp


  • Please log in to reply
No replies to this topic

#1 cmantz

cmantz

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 24 October 2010 - 07:40 AM

Hi,

I'm very new to QPCR (and to this forum!). I ran a QPCR plate the other day with 4 genes, one of which was GAPDH. And I don't know what happened but most of my samples did not return a Cp number for GAPDH but did for the other 3 genes. What could have been the cause of this?

Also, I don't know if this has already been posted but can someone explain to me what is the Cp? I know what the Ct is and I understand the 2^(Cp-Ct) concept but I don't understand the Cp and if I have values of Cp=40, why does this mean there is no gene present? Very confused... HELP!

Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.