I have been isolating rna manually from leaves of arabidopsis plants using phenol/sds method and using licl as additional step for precipitation of rna. I had to precipitate the rna in 2M Licl in 4C but i did it at -20C for overnight . I wonder how rna precipiates in -20C when the solution became ice.
how does rna precipitate in LiCl solution ?
1 reply to this topic
Posted 25 October 2010 - 08:18 AM
Lithium and other cations help to "shield" or neutralize negative charges on RNA/DNA. Time and temperature probably only effect yield. o/n precipitation at either temperature probably isn't necessary. More discussion here: