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how does rna precipitate in LiCl solution ?


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#1 deespike

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Posted 23 October 2010 - 03:32 AM

HI all

I have been isolating rna manually from leaves of arabidopsis plants using phenol/sds method and using licl as additional step for precipitation of rna. I had to precipitate the rna in 2M Licl in 4C but i did it at -20C for overnight . I wonder how rna precipiates in -20C when the solution became ice.


Please help.

#2 David C H

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Posted 25 October 2010 - 08:18 AM

Lithium and other cations help to "shield" or neutralize negative charges on RNA/DNA. Time and temperature probably only effect yield. o/n precipitation at either temperature probably isn't necessary. More discussion here:

http://www.madsci.or...55056.Mb.r.html

http://www.ambion.co.../tb/tb_160.html




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