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Large salt pellet after RNA precipitation won't go away


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#1 Jebra

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Posted 22 October 2010 - 08:39 AM

I was trying to concentrate my RNA bulk, doing the normal 1/20th sodium acetate + 2.5X ethanol.

Except this time, right after I added the ethanol all my salt crashed out and turned milky. It is NOT because of a high RNA yield, it is obvious the pellet is all salt. I spun it down, and washed the pellet with 70% ethanol FOUR times now. However, the salt pellet is still huge.

Any suggestions?





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