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RNA primed PCR


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#1 jessh

jessh

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Posted 20 October 2010 - 08:01 PM

Hi All,

I am wondering if anyone has any thoughts on whether using a RNA primer for standard PCR wouldn't work?? I am concerned about the heat stability of the RNA primers and I can't seem to find any literature where others have used an RNA primer in a PCR.

I want to use a RNA primer because I need to get rid of all excess primer so I figured I could use a RNase after the PCR to remove. I have tried many other ways to get rid of excess primer (i.e. biotin/streptavidin, columns, gels etc), however, the technique I am trying to work up is extremely sensitive so none of these have worked well enough and there is always carry over.

Thanks in advance,
Jess




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