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blood red cells


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#1 hellashellas

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Posted 20 October 2010 - 02:07 AM

Hi guys! I'm a new member of this forum. It's amazing. I have a question for you.
My lab has a collection of dried human blood (nucleated red blood cells) samples on Whatman cards. We are interested in extracting total proteins to be used in ELISA assay. Do you know if there is some protocol for this? I'd like to ask you if it's a good idea or not.
Thank you very much

#2 Clare

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Posted 20 October 2010 - 12:53 PM

Hi and welcome to BioForum :D

I doubt you'll get protein from those samples, DNA is tough, but proteins ain't.

Clare

Hi guys! I'm a new member of this forum. It's amazing. I have a question for you.
My lab has a collection of dried human blood (nucleated red blood cells) samples on Whatman cards. We are interested in extracting total proteins to be used in ELISA assay. Do you know if there is some protocol for this? I'd like to ask you if it's a good idea or not.
Thank you very much






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