Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ds cDNA synthesis problem


  • Please log in to reply
No replies to this topic

#1 doudou30

doudou30

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 19 October 2010 - 01:14 PM

Hi !

I am trying to do dscDNA from Immortalized Fibroblasts using Illumina kit. RNA is extract with trizol, purified with QIAgen column, and treated with DNase I. We found no degradation and no contamination (Nanodrop and Agilent) of the RNA. Then we try to do fs and dscDNA and check new synthetized DNA with nanodrop. Profiles are awfull :( . We are using the same protocol for Immortalized Lymphoblasts and it's working very well.
We tried differents concentration of RNA, but it didn't worked.

SO if anybody has an idea of how I could do it will be very helpfull !! :lol:

THanks a lot in advance !




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.