Lysis buffer selection
Posted 19 October 2010 - 12:03 PM
There are a lot of different recipes for a lot of different lysis buffers. Does anyone have a flow chart or general rules of thumb about which lysis buffer to choose? Does it depend on the type of cells that are being prepared (tissue or cell culture), the protein of interest, protein determination assay, antibody, etc? Any help will be GREATLY appreciated! Thanks.
Posted 19 October 2010 - 03:22 PM
Generally the more detergent and the less salt (i.e. more hypotonic) a lysis buffer has in it, the stronger it is.
Posted 29 October 2010 - 08:14 PM