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Primers no longer work


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#1 DrAnnie

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Posted 19 October 2010 - 11:18 AM

I have some PCR reactions that used to work like gold and now I'm getting nothing but high molecular weight smears. I know I could order new primers, but has anyone had any success with snap-freezing or any other primer saving technique? If you have a good protocol let me know.

#2 David C H

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Posted 20 October 2010 - 05:44 AM

You can try heating them at 95 deg. for 5 minutes, then put them on ice. Primers degrade over time, especially with multiple freeze/thaw cycles. Primers are cheap these days -- no reason not to order new primers.




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