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Specific activity


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3 replies to this topic

#1 lyok

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Posted 19 October 2010 - 06:20 AM

Dear all,

I have been using the website http://www.brenda-enzymes.org and I have been looking up some information on certain enzymes, I do however have a question about the "SPECIFIC ACTIVITY [Ámol/min/mg)". This is one of the parameters the website gives you, but what does this mean?

I havent had a lot of biochemistry and cant find any information about it that I understand.

#2 rkay447

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Posted 19 October 2010 - 06:53 AM

This is a measurement of how much product is formed by an enzyme in a given amount of time per mg of enzyme . I use specific activity for kinase reactions and what this means is that my kinase is capable of transferring X umol phosphate onto a substrate in a certain amount of time given a certain amount of active kinase. Usually a pure enzyme gives a constant number so this is also a good measurement of an enzyme's purity.

#3 lyok

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Posted 25 October 2010 - 01:09 AM

Thank you very much rkay447

#4 Inmost sun

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Posted 28 October 2010 - 05:41 AM

the specific activity is especially important if you work with non-pure or crude enzyme preparations




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