not completed transfer proteins on NT membrane
Posted 19 October 2010 - 04:23 AM
After I transfered, I found proteins some times completed transfering
but most of experiments the protein often detached. (see pic.)
I don't know why ?? what is the critical process ?
How should I do ?
Posted 19 October 2010 - 11:55 AM
(to oversimplify) keep in mind that the larger the protein, the less complete the transfer.
Edited by mdfenko, 19 October 2010 - 11:56 AM.
genius does what it must
i do what i get paid to do
Posted 26 October 2010 - 10:15 PM
This time, I extended the time for soaking the membrane (and also changed from 20%MeOH in water , the recommended from data sheet, to Transferring buffer only ). The result was nice
Thank you very much