RNA Pol II CHIP protocol help
Posted 18 October 2010 - 10:39 PM
I am trying to standardize RNA Pol II chip in the lab. I have tried 2 different protocols, but when i check the enrichments after pcr I see similar intensity bands in the no antibody control and the antibody positive sample, also i sonicate in 0.1% sds lysis buffer and in this condition even after 80 pulses i cant get rid of the >800bp fragments although 60% of the DNA is fragment size is from 500 to 100 bp.
can anyone suggest me a protocol that is standardized for RNA PolII chip. and if i can use a different lysis buffer for sonication?
Posted 19 October 2010 - 09:21 PM
Posted 22 November 2010 - 08:09 AM
any help would be appreciated about RNA Pol II's standardization.