Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA Pol II CHIP protocol help


  • Please log in to reply
2 replies to this topic

#1 Ananya Sadhu

Ananya Sadhu

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 18 October 2010 - 10:39 PM

Hi
I am trying to standardize RNA Pol II chip in the lab. I have tried 2 different protocols, but when i check the enrichments after pcr I see similar intensity bands in the no antibody control and the antibody positive sample, also i sonicate in 0.1% sds lysis buffer and in this condition even after 80 pulses i cant get rid of the >800bp fragments although 60% of the DNA is fragment size is from 500 to 100 bp.
can anyone suggest me a protocol that is standardized for RNA PolII chip. and if i can use a different lysis buffer for sonication?
thank you
ananya

#2 Ananya Sadhu

Ananya Sadhu

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 19 October 2010 - 09:21 PM

can anyone share a RNA pol II chip protocol i need it urgently!!

#3 sameerbau

sameerbau

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 22 November 2010 - 08:09 AM

same here.am trying to standardize the ChIP protocol using Ez-ChIP's method.
any help would be appreciated about RNA Pol II's standardization.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.