Hi,
very new to this thing and need help,
is there anyone know how to do 6.7%Polyacrylamide/50% Urea gel
Many thanks.
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1 reply to this topic
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mdfenko
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an elder
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Excellent
Excellent
Posted 18 October 2010 - 12:43 PM
what buffer are you using? what crosslinker concentration?
to 50gm urea, add buffer and acrylamide (with crosslinker) concentrates and water to 100ml.
stir (without heating, urea will decompose) untill all the urea is dissolved (it will take some time because the temperature will drop).
to 50gm urea, add buffer and acrylamide (with crosslinker) concentrates and water to 100ml.
stir (without heating, urea will decompose) untill all the urea is dissolved (it will take some time because the temperature will drop).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
