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6.7%Polyacrylamide/50% Urea gel


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#1 drchewan

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Posted 18 October 2010 - 07:40 AM

Hi,

very new to this thing and need help,

is there anyone know how to do 6.7%Polyacrylamide/50% Urea gel

Many thanks.

#2 mdfenko

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Posted 18 October 2010 - 12:43 PM

what buffer are you using? what crosslinker concentration?

to 50gm urea, add buffer and acrylamide (with crosslinker) concentrates and water to 100ml.

stir (without heating, urea will decompose) untill all the urea is dissolved (it will take some time because the temperature will drop).
talent does what it can
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