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NFKb signalling in cell line ???


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#1 GNANA

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Posted 18 October 2010 - 03:56 AM

Hi everybody,
i got to check whether the NFKb signalling is active in a group of cell lines....is looking at the IKB-alpha (western blot) is right way to guess whether NFKb is active or not?..is there any other way to asses the active NFKb signalling in cell lines?...thanks in advance for your suggestion.

Gnana..

Edited by GNANA, 18 October 2010 - 03:57 AM.

I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#2 ksturm

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Posted 18 October 2010 - 07:14 AM

IkappaBalpha is a good way. Another way: Activation of NF-kappaB leads to its phosphorylation. Use specific anti-phospho-p65 antibodies (example), you can proof whether NF-kappaB is activated or not in a more direct way.

#3 GNANA

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Posted 19 October 2010 - 05:21 AM

thats cool thank you ksturm...

Gnana..,

Edited by GNANA, 19 October 2010 - 05:22 AM.

I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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