Migration Distance on a Gel
Posted 17 October 2010 - 11:12 AM
Posted 17 October 2010 - 04:31 PM
Posted 20 October 2010 - 08:15 PM
If you want to work out the exact band size of your unknown bands you can use an old program called SEQAID. Its an old MS-DOS based program from 1991 but it still works well! It calculates the length (in bp) of your unknowns. You just need to put in the size (bp)and mobility (i.e. length in cm) for each band in your ladder and then the mobility of your unknowns and it determines the band size. The output is very basic you could easily put the numbers into excel if you wanted a graph.
You can download it from here: