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gst pulldown


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2 replies to this topic

#1 soymilk14

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Posted 17 October 2010 - 07:02 AM

hi everyone,

I have purified my fusion protein and is now conjugated with gst beads. MY question is, how long can i keep these fusion protein beads? Or should i do gst pulldown immediately?

Thank you.

#2 rkay447

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Posted 19 October 2010 - 01:57 PM

You can simply re-suspend your beads in a 50/50 mix of ethylene glycol/buffer and store them indefinitely at -20 degree. I do large scale purifications, quantify protein by coomassie of serial dilutions comparing to BSA standard, aliquot and store. I've been using the same purified proteins on gst beads for over 4 years with no problems. Just be sure to wash your beads a couple of times with buffer before doing the pulldown to remove all the ethylene glycol.

Edited by rkay447, 19 October 2010 - 01:59 PM.


#3 soymilk14

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Posted 21 October 2010 - 12:02 AM

thanks for the reply! I highly appreciate it..:)




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