Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Km values in enzymology

  • Please log in to reply
1 reply to this topic

#1 chn09



  • Active Members
  • PipPipPipPipPip
  • 44 posts

Posted 16 October 2010 - 08:35 PM

hello everyone

I would like to know whether it is possible to compare Km values of a alkaline phosphatase enzyme which i have purified (recombinant, expressed and purified from E. coli BL21 using afiinity chromatography) with other Km values in published references.Does Km change with the purification method?
Any suggestions please.

#2 Inmost sun

Inmost sun


  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 326 posts

Posted 17 October 2010 - 03:15 AM

comparing enzymatic activity of native enzyme and of bacterial, overexpressed cloned enzymes may be different; comparison is only preliminary

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.