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Km values in enzymology


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#1 chn09

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Posted 16 October 2010 - 08:35 PM

hello everyone

I would like to know whether it is possible to compare Km values of a alkaline phosphatase enzyme which i have purified (recombinant, expressed and purified from E. coli BL21 using afiinity chromatography) with other Km values in published references.Does Km change with the purification method?
Any suggestions please.

#2 Inmost sun

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Posted 17 October 2010 - 03:15 AM

comparing enzymatic activity of native enzyme and of bacterial, overexpressed cloned enzymes may be different; comparison is only preliminary




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