Hi everybody,
could anybody suggest me how to find whether the NFKb signalling is active in a cell line or not???
Gnana...,
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2 replies to this topic
#1
GNANA
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Posted 15 October 2010 - 01:56 PM
I always had an alternate hypothesis....
#2
microRNAboy
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Posted 28 October 2010 - 04:42 AM
Could you try transfecting in an NFKb reporter vector? http://www.promega.c...849/9pie849.pdf
The above vector has an NFKb response element fused with firefly luciferase. The response element can be activated by stimulating your cells (eg with TNFa) and then you read the luciferase expression.
The above vector has an NFKb response element fused with firefly luciferase. The response element can be activated by stimulating your cells (eg with TNFa) and then you read the luciferase expression.
#3
NFkb
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Posted 16 February 2011 - 03:24 AM
Hi,
NFkB is a complex of various subunits and it is responsible for more than 200 gene expression.
Can you be more specific on what you are trying to find out about ?
Which tissue.....
easiest would be to see phosphorylated status of p65 subunit with ELISA based method.
As p65/p50 is reported to be most active and naturally occurring NFkB subunit
Milan
NFkB is a complex of various subunits and it is responsible for more than 200 gene expression.
Can you be more specific on what you are trying to find out about ?
Which tissue.....
easiest would be to see phosphorylated status of p65 subunit with ELISA based method.
As p65/p50 is reported to be most active and naturally occurring NFkB subunit
Milan
