Hello All, I am a first year graduate student, just started my rotation. I am breaking open yeast cells to get mitochondria out, and I always start by digesting my yeast cell walls with zymolyase. After digestion, when I use glass beads to break open the cells it works perfectly and almost all cells are broken. However, for another protocol I have to break open the cells with 15 strokes of dounce homogenizer/glass potter. Regardless of how many strokes I do, these yeast do not want to break open!!! I feel really dumb, is there any trick to using these devices? I picked a glass/teflon combinaiton where the 'hammer' fits in really tight.
Thanks very much for any ideas!
Jason.
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