Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Dounce homogenizer issues!

  • Please log in to reply
No replies to this topic

#1 Jason2010



  • Members
  • Pip
  • 1 posts

Posted 13 October 2010 - 12:37 PM

Hello All, I am a first year graduate student, just started my rotation. I am breaking open yeast cells to get mitochondria out, and I always start by digesting my yeast cell walls with zymolyase. After digestion, when I use glass beads to break open the cells it works perfectly and almost all cells are broken. However, for another protocol I have to break open the cells with 15 strokes of dounce homogenizer/glass potter. Regardless of how many strokes I do, these yeast do not want to break open!!! I feel really dumb, is there any trick to using these devices? I picked a glass/teflon combinaiton where the 'hammer' fits in really tight.
Thanks very much for any ideas!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.