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sh design and cloning


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#1 erini

erini

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Posted 13 October 2010 - 06:48 AM

Dear

could you tell me your experience with TAKARA ligation kit (vs T4 ligase of NEB)? Have you experienced problems with cloning of sh into large vectors (100nt vs 10kB)?
Also, which is the prefered software these days for sh design?

thanks for your replies!




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