2 replies to this topic

[Irene]

Hi, I want to fix HEK293-YFP cells to observed them in confocal microscopy. I also need to use a DAPI staining to observe the nuclei, so I wonder if I could follow a normal immunofluorescence protocol, fixin cells with 4% PFA and permebealize with 0,1 % Triton x100.

thanks¡¡

[APoreUT]

You should be able to I think.  We typically fix, permeabilize, do any immuno/staining procedures, then add vectashield with DAPI.  This of course has wash steps between each of those.

[Irene]

Thank you¡¡ I will try in that way¡¡