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Fixing YFP cells


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#1 Irene

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Posted 13 October 2010 - 03:55 AM

Hi, I want to fix HEK293-YFP cells to observed them in confocal microscopy. I also need to use a DAPI staining to observe the nuclei, so I wonder if I could follow a normal immunofluorescence protocol, fixin cells with 4% PFA and permebealize with 0,1 % Triton x100.

thanks 

#2 APoreUT

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Posted 13 October 2010 - 03:59 AM

You should be able to I think. We typically fix, permeabilize, do any immuno/staining procedures, then add vectashield with DAPI. This of course has wash steps between each of those.

#3 Irene

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Posted 14 October 2010 - 05:39 AM

Thank you  I will try in that way 




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