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Strange SDS gel after Western Blotting


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#1 Off the Leish

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Posted 13 October 2010 - 12:35 AM

Could someone have a look at the picture of the SDS-PA gel below? It shows the gel after recombinant protein samples were electrophoresed then blotted onto a PVDF membrane for a Western. The gel was then Coomassie stained/destained. We would normally expect just the one ~42kDa band (possibally with a few contaminant bands) in the middle of the gel, which is there, but there is a strange speckling throughout the entire gel.

The Western Blot result is also shown and makes no sense either (but that's most likely a separate issue).

Cheers,

Patrick

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#2 mdfenko

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Posted 13 October 2010 - 10:11 AM

staining of the gel, this is normal after transfer (i think it may be caused by the texture of the filter paper).

i can't make out the blot (too dark).

Edited by mdfenko, 13 October 2010 - 10:12 AM.

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