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Strange infection In EPC cells


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#1 APoreUT

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Posted 12 October 2010 - 06:40 PM

Recently, while culture my cells I noticed spheres with little moving things in them. It almost seems as if something invaded the cell, caused it to detach fro the plate and used it to grow. I tried treating these cells with Ampicilin and gentimycin (and the have pen/strep in the media). This seemed to be working at first, but I left them alone for a few days and came back to see something wrather strange. I tried taking pictures at 40x (+10x optical for a total of 400x) with a phase contrast microscope. Unfortunately, given the multi-dimensionality of the infection it was hard to capture what I was seeing. There seemed to be tangles of what looked like hair and a few longer strands swimming accross the viewing area from time to time. I'm not sure where to even start with this. One of my friends believes this is protozoan, but I'm leading more towards a bacillus infection.

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Thanks in advance,
APoreUT

#2 bob1

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Posted 12 October 2010 - 09:04 PM

It is either that you have fluff in your culture or that you have fungal contamination.

#3 APoreUT

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Posted 13 October 2010 - 03:22 AM

It isn't "fluff" because it is growing" and it the fact that it moves also rules out being fluff. As far as I know, fungal infections can't swim through the dish as smaller stick-like shapes?

#4 bob1

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Posted 13 October 2010 - 03:15 PM

fungus then

#5 APoreUT

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Posted 13 October 2010 - 04:26 PM

fungus then


Fungus doesn't swim/move...

#6 bob1

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Posted 14 October 2010 - 05:10 PM

It does if it is floating, just as dead cells do - brownian motion and/or currents in the fluid.

Flagellate bacteria can also move by, but the thing you showed is too big for a bacterium.

#7 leelee

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Posted 14 October 2010 - 05:58 PM

I agree- looks like you have fungal contamination.




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