Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA isolation from human serum/plasma


  • Please log in to reply
1 reply to this topic

#1 kitty03_1978

kitty03_1978

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 12 October 2010 - 01:00 PM

Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...

I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!! ;)

#2 Montys

Montys

    member

  • Active Members
  • Pip
  • 16 posts
1
Neutral

Posted 18 October 2010 - 05:02 AM

Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...

I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!! ;)


I would just do an ethanol precipitation just as you would do with PCR reactions. Maybe a PCR-prufication Kit works also. You should just try.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.