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Total cell extracts for elisa assay


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#1 yo4trad

yo4trad

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Posted 12 October 2010 - 12:01 PM

What Lysis buffer works best for whole cell extracts for elisa! Is it ok to use 50mM Tris, 150mM NaCl, 1%NP40.

Does using NP40 have an effect on your samples?

Also, is it ok to dilute protein extracts in lysis buffer?

Thanks




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