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Viral DNA extraction


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#1 desertrose

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Posted 12 October 2010 - 03:03 AM

Hi bioforumers
Iam startig to wrk in a new project,my first task is to do PCR from lyophilized varicella vaccine (Oka strain)
Should I do direct PCR or DNA extraction and which protocol should I use

#2 bob1

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Posted 12 October 2010 - 08:59 PM

DNA extraction should give better (as in more consistent) results. A standard phenol-chloroform preparation should work well.




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