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Calculating the size of E. coli chromosome?


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#1 lolcakes

lolcakes

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Posted 12 October 2010 - 12:31 AM

We conducted an experiment where we used restriction endonuclease digestion of DNA. the results after electrophoresis show the plasmid DNA in discrete bands but the chromosomal DNA is a smeared profile. The question is to design a variation of the experiment that will enable you to calculate the size of the E.coli chromosome given that you were only given two enzymes that is a 4 base and 6 base cutter.




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