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Targeted capture sequencing


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#1 dna_nerd

dna_nerd

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Posted 11 October 2010 - 02:09 PM

Hello all

I am attempting to put together a proposal that involves sub-genomic sequencing experiments and need some help. Here is how I envision it working:

I would like to take 40-50 primary tumors and extract genomic DNA, create a custom array chip that will capture my regions of interest (three well-characterized genes that are 50-100kb in length).

Sequence these regions across all tumors and look at the genetic variation present therein.

I am hoping to use a GS Junior to do the sequencing (that works right?) and they recommend NimbleGen capture arrays.

Does this sound feasible??

Much obliged!




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