Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Targeted capture sequencing

  • Please log in to reply
No replies to this topic

#1 dna_nerd



  • Active Members
  • Pip
  • 27 posts

Posted 11 October 2010 - 02:09 PM

Hello all

I am attempting to put together a proposal that involves sub-genomic sequencing experiments and need some help. Here is how I envision it working:

I would like to take 40-50 primary tumors and extract genomic DNA, create a custom array chip that will capture my regions of interest (three well-characterized genes that are 50-100kb in length).

Sequence these regions across all tumors and look at the genetic variation present therein.

I am hoping to use a GS Junior to do the sequencing (that works right?) and they recommend NimbleGen capture arrays.

Does this sound feasible??

Much obliged!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.