I will be using G418 on HeLa cells. G418 concentrations of 0, 300, 400, 500, 600, 700 ug/mL
Should I change the G418-laced media every day? If so, can I just make individual batches of G418 at the different concentrations and keep those for a couple weeks? Or will the G418 be deactivated if I do it in bulk like this (ex. a vial of 300 ug/mL, a vial of 400 ug/mL...)
I'm not sure what concentration of CELLS to use in the wells in which I will be subjecting them to G418
For a "kill curve," is it necessary to actually make a curve? Or just look for what concentration gives death in the 4-5 day region.
Thanks a ton
Edited by Chemist, 11 October 2010 - 12:25 PM.