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Extraction protein from paraffin-embedded tissues


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#1 DoD

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Posted 11 October 2010 - 04:33 AM

Hi

I would like to quantify some proteins in paraffin-embedded tissues. I have no idea how I can extract the proteins and quantify them.

I would like to stard with a 10Ám slide. I think I can start with a classical HIC protocol to remove the paraffin but after that???? I have no idea. No one.

If someone can help me I will be more than happy

Thanks

#2 newarray

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Posted 28 December 2010 - 12:12 PM

I would check the protein by immunofluoresence or IHC.If its not feasible then extract the cells and check the proteins by Western analysis.




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