real time PCR machine
Posted 10 October 2010 - 08:47 AM
so my supervisor is trying to apply for a grant to get a real time PCR machine, and we've been asking for quotes from several different companies. I just started using real time machine pretty much a week ago and really green in knowing of what to get. There are so many of them and each company have different products. I am wondering if you can recommend me of what would be the most robust and easiest machine to get? We need them particularly for gene expression study, so I don't think we need those multiplexing features and all that. I heard that in biorad iQ5 you need to calibrate the machine everytime the volume of reaction changes. Is that true? I am using ABI 7500 now and it doesn't seem that I need to do so, I just need to tell the software the volume of reaction. Nevertheless Biorad iQ5 seems to be pretty popular. Anyhow, if anyone can advice on what features to look for, I'd greatly appreciate it!
Posted 10 October 2010 - 09:22 AM
Posted 01 November 2010 - 03:30 PM
Posted 27 March 2013 - 03:39 AM
We have PikoReal in the lab from ThermoFisher. fairly cheap and simple. Plates are cheap as well as they are only 1/4 size and still has 96 reactions. The problem i saw is that the program is slow (u need newer computer for it compared to ABI products) and the white plates are VERY annoying if you use transparent master mix (we used DynamoColor flash, now Thermofisher offers Luminaris blue master mixes - I want to try those).
Rotorgene... well i dont like it personaly - need to cap all the tubes (4 at the time) - its time consuming, and i dont have long pipete tips for them so this machine is kinda used by the people who are worried of NTC going up. Buth the good thing is - the cycles can be VERY short (i got down to 4 seconds) good for some kinetics experiments if you can make them work in qPCR.
Posted 02 August 2014 - 06:10 AM
I need to do calibration for StepOne of ABI. Can anyone help me to know how to do it without using the company's kit because the kit is so expensive? Thanks.