Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Making solutions for RNA work


  • Please log in to reply
1 reply to this topic

#1 Anemone_2010

Anemone_2010

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 09 October 2010 - 03:42 PM

Hello, I am about to do a Northern blot but I am still hesitated to use the ph meter for making solutions since it is a shared equipment, it may have some RNase on it. I cannot simply add DEPC to the solution and autoclave because it contains Tris. Anyone has worked with RNA and made&pH solutions successfully? Thanks in advance for any suggestions!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,603 posts
385
Excellent

Posted 10 October 2010 - 03:32 PM

Ideally what you would do is take a small volume out and pH it, after each addition of acid. The other option for pHing tris solutions is to make them up with tris base and tris HCl crystals in the required ratio to give a the final pH wanted.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.