gDNA restriction digest
Posted 08 October 2010 - 07:44 AM
I'm a bit confused. I'm attempting to perform a partial restriction digest of gDNA. but What should this look like on a gel? i want bands between 1kb-12kb. Should it just be a a smear in my size range? And Should there be undigested gDNA visible? Also what would a complete digestion of the gDNA look like?I am restricting with EcoRI
Many thanks for your time!
Posted 08 October 2010 - 09:02 AM
Normally you have to try out which enzyme(s) produce the range of fragment sizes you need. Or you find a publication with some information about this.
Edited by hobglobin, 08 October 2010 - 09:18 AM.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.