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why most Annexin 5 products are conjugated?


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7 replies to this topic

#1 Curtis

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Posted 08 October 2010 - 06:24 AM

I want to buy an Annexin 5 from Sigma but most products and kits got conjugated Annexin (e.g -FITC). I thought Annexin stains cells on its own like how Propidium Iodide does. I need to use it for flow cyto and don't know which one to buy.

#2 Rsm

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Posted 09 October 2010 - 08:18 AM

Annexin 5 is the surface epitope that is recognized by the anti-Annexin 5 antibody you want to buy. That antibody should be conjugated, if you want to use it for FACS. I'd recommend APC conjugation, if you want to use PI as well... However, FITC should be working as well.

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#3 Curtis

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Posted 10 October 2010 - 10:53 AM

um...Annexin 5-FITC is not antibody, is it?

#4 Rsm

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Posted 10 October 2010 - 10:33 PM

No, Annexin 5 is the surface epitope that is recognized by the anti-Annexin 5 antibody, which is conjugated to FITC.
http://www.biocompar...6-18937&sb=true

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#5 Curtis

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Posted 11 October 2010 - 12:35 AM

I'm talking about fluorescent dye labeled annexin v:

http://www.sigmaaldr...RAND_KEY&F=SPEC

#6 Rsm

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Posted 11 October 2010 - 04:23 AM

Oh, you're right, I didn't know that there was this distinction. However, you still need a method of detection of Annexin 5 binding. How do you want to do it, if not by fluorescent labelling?
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#7 Curtis

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Posted 11 October 2010 - 10:36 AM

most probably Annexin 5 is useless on its own so that's why they label it.

#8 moljul

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Posted 12 October 2010 - 12:34 AM

...i thought that annexin-5 recognizes phosphatidylserin which swiches onto the outer leaflet of the cell-membrane during apoptosis process!

annexin 5 is a phospholipid-binding protein (NO antibody) therefore it has to be labelled!!




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