Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cell cycle arrest by colcemid


  • Please log in to reply
2 replies to this topic

#1 ija0

ija0

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 08 October 2010 - 01:52 AM

I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?

#2 aleruiz

aleruiz

    member

  • Active Members
  • Pip
  • 18 posts
0
Neutral

Posted 13 October 2010 - 07:12 AM

I've never used Colcemid, but I use Mitomycin C in medium. Could you use it as an equivalent?


I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?



#3 Denis Baev

Denis Baev

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
1
Neutral

Posted 25 January 2011 - 05:55 AM

I stain my cells with CFSE to analyze their cell cycle activity

???? Cell cycle by CFSE? :)
May be "to analyse cell divisions"?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.