Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

proteinase k inhibition/inactivation


  • Please log in to reply
2 replies to this topic

#1 rick112

rick112

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 71 posts
0
Neutral

Posted 07 October 2010 - 09:17 PM

hi

i am working on a RT-PCR method...i have used "proteinase k" for treatment of sample,tried heat inactivation method the proteinase k but resulted in precipitation of my samples.
could anyone tell me other methods for Proteinase K inhibition/inactivation.

#2 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,199 posts
109
Excellent

Posted 08 October 2010 - 06:43 AM

It's hard to tell anything when you didn't described what exactly are you doing (what protocol) and why.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,453 posts
245
Excellent

Posted 08 October 2010 - 01:40 PM

PMSF will inhibit proteinase-K. It's pretty toxic, and not stable in water, so you have to prepare solutions (in isopropanol) fresh. Careful.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.