Posted 07 October 2010 - 06:32 AM
Posted 08 October 2010 - 07:46 AM
one factor may be uneven distribution of sds on the protein. this will cause the charge density to be different from expected and will cause the protein to migrate at a different speed than other proteins. i suspect that this is the cause of your confusion.
to get a better estimate you can run your protein on gel filtration liquid chromatography.
to get the actual molecular weight you can use lc-ms.
Edited by mdfenko, 08 October 2010 - 07:46 AM.
genius does what it must
i do what i get paid to do