i am in a confusion how to interpret my result. i explain wat i did, my aim is to identify the signalling through which my gene is being made express (which is an overexpressed gene in cancer). to do this i treated the cancer cells with different inhibitors of signalling, in that i found the NFKb inhibitor has reduced my genes expression with respect to time, i mean i could see a gradual decline in the expression in course of time (on SDS PAGE),,,also this drug kills cell as well, my time points are from 30 mins to 6 hrs with 10 micromolar conc of the inhibitor. i even normalized with tubulin and PhosphoMAPK both seems to be unchanged and well quantified. so with this can i say the sharp decline in the expression of my gene of interest is drug induced or still it is bcoz of dying cells. shd i hav to do any other assay to confirm this result ??? ur suggestions will help me a lot. thanks in advance.
Edited by GNANA, 06 October 2010 - 11:27 PM.