Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

is my lost protein is bcoz of cell death or my treatment worked???


  • Please log in to reply
2 replies to this topic

#1 GNANA

GNANA

    GNANA

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 207 posts
9
Neutral

Posted 06 October 2010 - 11:25 PM

Hi everybody,
i am in a confusion how to interpret my result. i explain wat i did, my aim is to identify the signalling through which my gene is being made express (which is an overexpressed gene in cancer). to do this i treated the cancer cells with different inhibitors of signalling, in that i found the NFKb inhibitor has reduced my genes expression with respect to time, i mean i could see a gradual decline in the expression in course of time (on SDS PAGE),,,also this drug kills cell as well, my time points are from 30 mins to 6 hrs with 10 micromolar conc of the inhibitor. i even normalized with tubulin and PhosphoMAPK both seems to be unchanged and well quantified. so with this can i say the sharp decline in the expression of my gene of interest is drug induced or still it is bcoz of dying cells. shd i hav to do any other assay to confirm this result ??? ur suggestions will help me a lot. thanks in advance.

Gnana..,

Edited by GNANA, 06 October 2010 - 11:27 PM.

I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#2 madrius1

madrius1

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 114 posts
0
Neutral

Posted 07 October 2010 - 09:20 AM

It seems rather unlikely that the decrease in expression you see is to be related with cell death, given that the two controls you have done are unchanged. But if you want to make sure that apoptosis is not the cause of the protein loss, you could blot for clived caspases or parp, which could monitor apoptosis.

#3 GNANA

GNANA

    GNANA

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 207 posts
9
Neutral

Posted 07 October 2010 - 11:59 AM

thanks a lot madrius, i try to execute ur suggestion.

gnana...
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.