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Determination of Primers for miRNA Primer Assay & miRNA Precursor Assay


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#1 happy@

happy@

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Posted 06 October 2010 - 08:09 PM

Hi all,

I am interested to amplify the precursor miRNA as well as the mature miRNA by RT-PCR. I would like to know where can i get a specified primer sequence (forward/reverse) for the miRNA that i would like to look into?

Actually i've referred to previous post at http://www.protocol-...es-for-cloning/ . Is this applicable for the RT-PCR primer?

Thanks.

Regards,
Happy@




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