Posted 05 October 2010 - 12:05 PM
I am preparing a expression vector and I would like to get your advice on glycine linker. I have a gene of interest and to over express and I have to insert a RFP gene somewhere middle of this gene to see the localization of our gene of interest. I am totally unsure about the glycine linker which I am supposed to add before and end of the RFP gene. Please advise me the points which I have to keep in mind regarding glycine linker, like number of glycine residues and what are the criteria for the selecting the numbers etc. Thanks in advance.
Posted 05 October 2010 - 06:07 PM
Posted 06 October 2010 - 01:44 PM
Thank you very much for your reply...
Usually linkers are a mix of glycine and serine residues, often the sequence GGGGSGGGGSGGGGS on the theory that the serines provide a bit of hydrophilic nature to the linker.